Screening of Lentinula edodes strains by laccase induction and resistance to Trichoderma sp.
DOI:
https://doi.org/10.33885/sf.1998.3.868Keywords:
induction, laccase, polyphenol, oxidases, Lentinula edodes, Trichoderma sp., antagonism, edible mushroomAbstract
Production of lacease (E.C. 1.10.3.2) and its induction by adding water soluble lignin derivatives obtained
from Indulin AT, were studied in two succesive cultures of 39 strains of Lentinula edades. Enzymatic activity
was measured by oxidation of ABTS in the second culture and the inductor effect on mycelial growth was
evaluated. The antagonism between a strain of Trichodertna sp. and 54 strains of L. edades was tested in
vitro. Resistance of L. edades to Trichoderma invasión was shown by the formation of a brown line at the
contact zone of the two fungí. The ability for lacease production of L. edades strains is not corresponding to
their ability for Trichoderma sp. réjection. This study shows diversity among L. edades strains that could be
applied to a program of selection and improvement
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