Characterization of methanolic extracts of vineyard pruning and wheat straw and Its oxidation by extracellular enzymes from Lentinula edodes
DOI:
https://doi.org/10.33885/sf.2013.3.1113Keywords:
shiitake, laccases, agricultural wastes, oxidationAbstract
Lentinula edodes is a mushroom of commercial interest which can be grown on several
lignocellulosic substrates, such as vineyard pruning and wheat straw. In this study we obtained
methanolic extracts from both of these substrates, and crude enzymatic extract from L. edodes.
The methanolic extracts were analyzed and characterized by thin layer chromatography and
spectrophotometry finding that vineyard pruning extracts contained at least six UV light-active
fractions; the three most polar fractions (R< 0.5) were easily oxidized by the fungal extract with f
or without ABTS intermediary. In contrast, the methanolic extract of wheat straw showed only
three fractions dimly revealed to UV light, which were not oxidized by the enzymatic extract
even using ABTS. The presence of a higher number or subfractions in vineyard pruning
methanolic extract, their possible higher concentration and the easily with which some of them
were transformed by the fungus extracellular enzymes would explain in part because vineyard
pruning is an excellent substrate for mushroom cultivation regarding to other substrates.
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