Production of laccase from Coriolopsis gallica at pilot plant scale
DOI:
https://doi.org/10.33885/sf.2010.3.1067Keywords:
fungal laccases, enzyme production, white rot fungiAbstract
The production of laccase from the ligninolytic fungi Coriolopsis gallica was carried
out at pilot plant scale in a 100 L bioreactor. A cheap fermentation medium was used
containing 3% Bran Flakes in 60 mM phosphate buffer, pH 6.0 containing 0.8 mM copper
sulfate. A total of 630 650 laccase units were obtained in 7-days cultures. After few
purification steps, including centrifugation, ultracentrifugation, ammonium sulfate
precipitation, and two ionic exchange chromatographies, a partially purified preparation was
obtained with 79% of laccase showing a specific activity of 313 U/mg.
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