Experimental cultivation of shiitake mushroom Lentinula edodes on two agricultural by-products from Guerrero, Mexico
DOI:
https://doi.org/10.33885/sf.2006.3.979Keywords:
Shiitake, Lentinula edodes, agricultural by-products, mushrooms cultivationAbstract
Mycelial growth of two strains (IE-40 and IE-105) of Lentinula edodes was evaluated
on plant of alfalfa, peanut hull, rice straw, sorghum straw and coconut fibres. The first two
agricultural by-products were utilized as substrates in an experimental cultivation. The
substrates were pasteurized in water at 75 ºC during 90 min and inoculated under nonaxenic
conditions. Spawn was prepared from sorghum grains, peat moss, gypsum and
powdered coffee pulp. In inoculation, plastic bags were utilized and incubated at 24 ± 1 ºC
in darkness for eight weeks. In fruiting period, the temperature was of 20 ± 1 ºC, relative
humidity between 85-90% and natural light 11 h per day. At three weeks of incubation, all
replicates of plant of alfalfa showed a contamination by bacteria and they were discarded. In
both strains one flush was obtained on peanut hull substrate. The primordia formation was
presented on average to 59.1 ± 1.72 and 61.1 ± 2.23 days after inoculation with strains IE-
40 and IE-105, respectively. The average at values of yield was of 291.64 ± 64.36 and 232.5
± 71.03 g of fresh mushrooms that are equal to 55.5 ± 12.23 and 44.2 ± 13.5% of biological
efficiency for strains IE-40 and IE-105, respectively. In the production there were not
statistical differences among strains. The peanut hull could be an acceptable substrate for L.
edodes production.
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