Growth and sporulation of Hirsutella thompsonii in natural media and the conservation of its viability in different supports
DOI:
https://doi.org/10.33885/sf.1992.3.794Abstract
The hyphomycete acarine pathogen, Hirsutella thompsonii, was
grown in liquid culture media composed of natural carbon and
nitr ogen sources cointained in wheat bran , potato-carrot, corn
meal and corn steep liquor , with or without 0 . 5 ml of Tween 80 and
incubated for 13 days at 26-28°C in a s haker bath at 70 hits;min.
Fungal biomass was determined by the mycelium dry weight, and
sporulation by number of conidia/ml. Biomass production was higher
in wheat bran, and in corn meal media with Tween 80. The higher
concentration of conidia ( 5.5 x 104 conidiafml ) was observed in
wheat bran medium without Tween 80 . The addition of Tween 80 did
not increase the production of conidia. The fungus was stored in
straw, wheat bran, sterile distilled water, physiological saline
solution, mineral oil or 10% glicerol, and in anhydrous silica gel,
at room and refr igeration temperatures . Low temperature was more
efficient in maintaining viability during the storage period
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