Using ITS sequences to assess intraspecific genetic relationships among geographically separated collections of the myxomycete Didymium squamulosum
DOI:
https://doi.org/10.33885/sf.2008.3.1024Keywords:
ITS, intraspecific variationAbstract
An analysis of ITS sequences of Didymium squamulosum, a myxomycete regarded
as cosmopolitan, shows variation among geographically separated isolates. ITS 1 and 2 and
5.8S rDNA were amplified from specimens collected in widely scattered localities. The
sequences were analyzed by parsimony analysis and the resulting trees show separate,
moderate and well supported clades grouping some, but not all, of the geographic locations.
ITS is the first molecular marker examined for intraspecific variation in myxomycetes, and
although sequence analysis shows statistically significant differences among sequences from
the 14 specimens considered, the heterogeneity in ITS 1 and ITS 2 appears to be too great for
any more meaningful biogeographical conclusions. Variation in the 5.8S and the amount of
variation found in ITS 1 and 2 may signify that the isolates examined represent a complex of
sibling species, although this particular marker cannot be used to make that distinction in this
myxomycete.
Downloads
Downloads
How to Cite
Issue
Section
License
Copyright notice
Open access policy
The authors who publish in this journal accept the following conditions:
In accordance with copyright laws, Scientia Fungorum recognizes and respects the authors’ moral rights, as well as the ownership of property rights, which will be transferred to the journal for dissemination in open access. Scientia Fungorum does not charge for submission and processing of articles for publication.
All the texts published by Scientia Fungorum –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The authors can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Scientia Fungorum (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Scientia Fungorum.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a complementary file.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license (CC-By-NC-SA 4.0).
