Isolation and regeneration of Rhynchosporium secalis protoplasts the causal agent of barley scald

Authors

  • Alfredo D. Martínez-Espinoza Centro de Investigación y de Estudios Avanzados, Departamento de Ingeniería Genética, Instituto Politécnico Nacional. Irapuato, Guanajuato, México
  • David C. Sands Department of Plant Pathology, Montana State University. Bozeman, Montana, E.U.A.

DOI:

https://doi.org/10.33885/sf.1995.3.836

Keywords:

Protoplasts, Rhynchosporium secalis, Scald

Abstract

Protoplasts from conidia and mycelium were isolated from the fungus Rhynchosporium secails by using Novozymc
234 at 10 mg/ml. The age of the culture was a determinant factor to obtain protoplasts from mycelium. but it did
not have a significant effect in the case of conidia. Shaking during incubation of the fungus with I be enzy1nc was
imponant in the production of protoplasts from mycelium, while it was not important for protoplast isolation from
conidia. Fluorescent microscopy revealed that the protoplasts were mainly uninuclcatcd 90-100% when obtained
from conidia. and 70 -80% uninucleated when obtained from mycelium. The remaining protoplasts bearcd two
nuclei. Ten percent of the total protoplasts were regenerated and gave rise to discrele colonies on the medium used.

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How to Cite

Martínez-Espinoza, A. D., & Sands, D. C. (2016). Isolation and regeneration of Rhynchosporium secalis protoplasts the causal agent of barley scald. Scientia Fungorum, 3(11), 155–164. https://doi.org/10.33885/sf.1995.3.836

Issue

No. 11 (1995): RMM núm. 11 1995

Section

Original papers

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