Development of a system to study spore germination in Aspergillus niger

Authors

  • Javier Barrios González Departamento de Biotecnología, Universidad Autónoma Metropolitana. México, D.F., México
  • Samuel Anaya Departamento de Biotecnología, Universidad Autónoma Metropolitana. México, D.F., México

DOI:

https://doi.org/10.33885/sf.1987.3.680

Abstract

A system to study fungal spores germination was developed. Spore germination
is performed i n shake flask and the ratio of germinated and ungerminated spores is
determined at different times by means of a Neubauer chamber. The kinetics of spore
germination thus obtained show a defined slope (germination rate) which is calculated
by linear r egression. When this method was used to study spores of Aspergillus
niger, the res ults obtained showed great variability. It is shown that this variability
is due t o the age of the culture used to inoculate the media where the spores
are produced. This effect is very intense when the spores are germinated in a
medium with s t arch as the carbon source. If new cultures are employed or spore ger
mination is per formed in a medium with glucose, variability is greatly reduced. Un~
der these cond i tions, the system shows great sensitivity

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How to Cite

Barrios González, J., & Anaya, S. (2016). Development of a system to study spore germination in Aspergillus niger. Scientia Fungorum, 3(3), 9–18. https://doi.org/10.33885/sf.1987.3.680

Issue

No. 3 (1987): RMM núm. 3 1987

Section

Original papers

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