Molecular identification and culture of Cordyceps brongniartii, a teleomorph of Beauveria brongniartii: A new record for Morelos, Mexico
Abstract
Background: There is a growing interest in the study of fungi throughout the world, with new species being described, as well as novel biological activities of already known species. The cultivation of the fungus Cordyceps militaris, the second most studied species of the genus Cordyceps in the world after C. sinensis, has seen great advances in the production of stromata in vivo. However, much less attention has been paid to other potentially cultivable species.
Objective: This work aims to identify wild specimens of the Cordyceps genus and to generate data on the production of cultured stromata.
Materials: To achieve the objectives, the following steps were followed: i. Collection and isolation of wild Cordyceps strains, ii. Identification and molecular analysis of the isolated species, iii. Stroma production in supplemented substrates.
Results and conclusions: Sequence analysis of the region ITS confirms the identification of C. brongniartii, an anamorphic state of Beauveria brongniartii, and this work provides the first report of the species in Morelos, Mexico. In addition, the in vitro production of fertile C. brongniartii stromata, obtaining fruiting bodies with characteristics like those of field-collected progenitors, with a biological efficiency of 36 %.
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