Morphologic reactivation of some dermatophyte species and their susceptibility to antifungal drugs
DOI:
https://doi.org/10.33885/sf.2015.3.1136Keywords:
Iitraconazole, terbinafine, Trichophyton mentagrophytes, Trichophyton rubrumAbstract
The preservation of fungi in collections frequently induces atypical changes of the specimens. In this work, several culture media were
evaluated in order to recover the morphological characteristics of dermatophytes preserved in average for 10 years and to determine
their susceptibility pattern to nine antifungal drugs. Fifty-four isolates were studied, from them 15 primary isolates and 39 from collection,
including the ATCC 9533 Trichophyton mentagrophytes strain. The majority of the isolates correspond to T. mentagrophytes, T.
rubrum, and T. tonsurans. All dermatophytes were firstly reactivated growing them in Sabouraud dextrose agar and then cultured on five
different media containing diverse ingredients to evaluate the species morphology. For the susceptibility studies, the broth microdilution
method was used, according to instructions of the document M38-A2 (Clinical and Laboratory Standards Institute). Most of collection’
dermatophytes initially showed colonies with atypical morphology. After their growth on different media, modified Gorodkowa agar,
oat-salts agar, and soil-hair agar induced numerous conidia production. In the last one, also perforating organs were formed. Terbinafine
inhibited 85 % of dermatophyte isolates (0.0008 and 0.03 μg/mL); caspofungin and anidulafungin showed low or null activity
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