Isolation and propagation of pure cultures of arbuscular mycorrhizal fungi from avocado orchards by the minirhizotron technique
DOI:
https://doi.org/10.33885/sf.2013.3.1110Keywords:
monospecific culture, infectivityAbstract
The aim of this study was to isolate arbuscular mycorrhizal fungi (AMF) from soils of
avocado orchards with different agricultural management and to propagate them through the
minirhizotron technique. Soil was extracted directly from the study areas, using a completely
randomized design and quantifying AMF species richness. Three of the most abundant species,
Rhizophagus aff. intraradices, Glomus sp. 1 and Sclerocystis rubiformis were propagated by the
minirhizotron technique, assessing mycorrhizal colonization after 20 and 40 days. This system
proved to be most effective for propagation and mycorrhization of Sclerocystis rubiformis
(18.5%) and Rhizhophagus aff. intraradices and Glomus sp. 1 (7.7%). Minirhizotrons with a
consortium of Glomus spp. and Rhizophagus. aff. intraradices and a monospecific culture of
Sclerocystis rubiformis were then transplanted to trap pots, for a period of four months. In the
minirhizotron culture, the consortium was highly infective from the early stages, reaching 95% of
mycorrhizal colonization in comparison with the pure culture of Sclerocystis rubiformis who
showed percentages of 58% at the end of the experiment. Therefore the minirhizotron system
used proved to be effective in the spread of mono- or multi-specific spore cultures in vivo.
Downloads
Downloads
How to Cite
Issue
Section
License
Copyright notice
Open access policy
The authors who publish in this journal accept the following conditions:
In accordance with copyright laws, Scientia Fungorum recognizes and respects the authors’ moral rights, as well as the ownership of property rights, which will be transferred to the journal for dissemination in open access. Scientia Fungorum does not charge for submission and processing of articles for publication.
All the texts published by Scientia Fungorum –with no exception– are distributed under a Creative Commons License Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0), which allows third parties to use the publication as long as the work’s authorship and its first publication in this journal are mentioned.
The authors can enter into independent and additional contractual agreements for the nonexclusive distribution of the version of the article published in Scientia Fungorum (for example include it into an institutional repository or publish it in a book) as long as it is clearly and explicitly indicated that the work was published for the first time in Scientia Fungorum.
For all the above, the authors shall send the form of Letter-transfer of Property Rights for the first publication duly filled in and signed by the author(s). This form must be sent as a complementary file.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license (CC-By-NC-SA 4.0).
